Characterisation of trypanosome isolates collected from cattle in Ghibe, Ethiopia. by Victorin Codjia

Cover of: Characterisation of trypanosome isolates collected from cattle in Ghibe, Ethiopia. | Victorin Codjia

Published by Brunel University in Uxbridge .

Written in English

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Thesis (M.Phil.) - Brunel University.

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ContributionsBrunel University. Department of Biology and Biochemistry.
The Physical Object
Pagination158p. :
Number of Pages158
ID Numbers
Open LibraryOL19720288M

Download Characterisation of trypanosome isolates collected from cattle in Ghibe, Ethiopia.

The study was conducted in the part of Omo-Ghibe tsetse belt located in Southern Nations, Nationalities and Peoples Regional State (SNNPRS). Out of 64 districts found in the study area, 11 were selected purposively for both parasitological and entomological studies based on the frequency of trypanosomosis case records obtained from district veterinary clinics and also opinion of by: 4.

Blood samples were collected from parasitaemic cattle in the Ghibe Valley, Ethiopia, frozen in liquid nitrogen and transported to Nairobi, Kenya. Twelve of the stabilates were inoculated into individual Boran (Bos indicus) calves and characterised for their sensitivity, in turn, to diminazene aceturate (Berenil), isometamidium chloride (Samorin.

1. Introduction. Traditionally, the various trypanosome species have been characterised by morphology and morphometrics, using stained blood smears, movement characteristics of the organisms in fresh blood films, localisation of the various developmental forms of the parasite in the tsetse fly, and the infectivity and virulence of the trypanosomes in various animal hosts (Hoare, Cited by: 3.

Trypanosome populations Field isolation Trypanosome field isolates were collected from male and female East African Zebu cattle at Ghibe in July into potassium ethylenediamine tetra-acetate (EDTA), and stored in liquid nitrogen (Dar et al. Thereafter, they were transported to Nairobi, Kenya, for by: Ten trypanosome isolates were collected at Ethiopia.

book from cattle at Ghibe, Ethiopia, in February and all shown to be savannah-type Trypanosoma congolense. The aim of this study is to characterize trypanosomes isolated from dogs and cattle in Jos South Abattoir. Isolated trypanosomes were subjected to polymerase chain reaction using kin primers targeting internal transcribed spacer 1 (ITS1) of ribosomal deoxyribonucleic acids.

The PCR allowed detection of three trypanosome livestock species namely. Koffi et Ethiopia. book. Appl. Biosci. Molecular characterization of trypanosomes isolated from naturally infected cattle in the "Pays Lobi" of Côte d’Ivoire ). AAT remains a major pathological constraint for livestock development in sub-Saharan Africa, in areas that have high forage and.

Baro, Akobo, Didessa, Ghibe and Omo. The most important trypanosome species affecting cat- tle in Ethiopia are. congolense, T. vivax. and. brucei. Trypanosomosis in cattle locally referred, Ethiopia. book “Gendi” is a serious constraint to livestock production in areas of the north and southwest Ethiopia at an altitude of below m.a.s.l [9].

A cross-sectional study to determine the prevalence of bovine trypanosomosis was carried out in Chena district, Kefa zone, southwest Ethiopia from September to January Blood samples were collected from randomly selected local (zebu) breed cattle in three representative peasant associations (PAs).

The buffy coat and Giemsa stained thin blood films examination techniques were. Gondar is located between 12°36′N and 33°28′E at an altitude of about meter above sea level with an average temperature of 20 °C and an average annual rainfall of mm. For the experimental study field isolates of trypanosomes were collected from Trypanosomosis prevalent areas of Birsheleko and Jawi in northwest Ethiopia.

Codjia et al. [18] reported thatonly one out of 12 trypanosome isolates collected from cattle in Ghibe were sensitive to mg/kg b.w. An overall failure rate for DIM in this finding was The four isolates of T.

congolense from Ghibe, Bedelle, Sodo and Arbaminch regions of Ethiopia were found to be resistant to the curative action of diminazene (in mice and cattle) and isometamidium (in cattle) at a dose rate of and mg/kg body weight, respectively (Chaka and Abebe, ). The findings indicated that tsetse flies and trypanosome infections of cattle are widely distributed in Ethiopia and their abundance is affected by spatial factors such as.

Ten trypanosome isolates were collected at random from cattle at Ghibe, Ethiopia, in February and all shown to be savannah-type Trypanosoma inoculated into naı̈ve Boran (Bos indicus) calves, all 10 isolates were resistant to diminazene aceturate (Berenil®), isometamidium chloride (Samorin®) and homidium chloride (Novidium®) at doses of mg/kg body.

Response of four indigenous cattle breeds to natural tsetse and trypanosomosis challenge in the Ghibe valley of Ethiopia. Veterinary Parasitology (1–2)– CrossRef PubMed Google Scholar Murray, M., Trail, J.C.M., Turner D.A.

and Wissocq, Y., congolense resistance originally isolated from cattle in the Southwest of Ethiopia, namely, Ghibe, Bedelle, Sodo and Arbaminch. In view of today’s resistance reports from many parts of Ethiopia including Ghibe valley, intervention needs to be complemented with regular surveillance of the evolution of the trypanocidal drug resistance.

of trypanosomosis is the main cause of decline in the number of cattle and particularly draught oxen (Abebe and Jobere, ). Report from the tsetse infested area of Ethiopia indicated that T.

congolense is the most prevalent trypanosome species (Abebe and Jobre, ; Rowland et al., ). Research has been undertaken since in the Ghibe valley, southwest Ethiopia, monitoring approximately village East African Zebu cattle monthly to assess the effects of trypanosomiasis on productivity.

The average monthly trypanosome prevalence between and was about 30. collected from cattle 19 Summary of location of traps, the number of flies caught and species of the tsetse flies 27 Complete culture medium for propagation of procyclic trypanosomes 28 Trypanosome isolates analyzed in the study 30 Oligonucleotide primers for amplification of DNA from different trypanosomes In experiments to characterise the uptake kinetics of [14C]-Samorin, the maximal rates of uptake (Vmax) for 4 Ghibe isolates ranged from to ng/10(8) trypanosomes/min.

infected by trypanosomes, and have good PCV, production and reproduction. A broader use of the Sheko breed in tsetse infested areas could improve animal health and household welfare. Immediate actions are needed to avoid extinction of this valuable breed.

Keywords: cattle, Ethiopia, trypanosomosis, trypanotolerance, phenotypic. The current study was undertaken from December to May with the aim of determining and comparing the pathogenicity and response to diminazene aceturate (DA) and isometamidium chloride (ISM) treatment in experimentally infected mice with trypanosome isolates from Jawi and Birsheleko areas of northwest Ethiopia.

A total of 42 mice were used for the experiment. The work was supported by the Overseas Development Administration of the United Kingdom.

This is ILRAD publication No. References Brun, R. and Rab, S. () In vitro drug sensitivity of Trvpanosoma congolense isolates. Parasitol. Res. 77, Codjia, V.

() Characterisation of trypanosome isolates collected from cattle in Ghibe. The incidence of new and the prevalence and persistence of recurrent trypanosome infections in cattle in southwest Ethiopia exposed to a high challenge with drug-resistant parasites May Acta.

A cross-sectional study was conducted from December to February to estimate the prevalence of bovine trypanosomosis and identify species of trypanosomes infecting cattle. Besides, attempt was made to assess the knowledge and practice of the livestock owners in Assosa district of Benishangul Gumuz Regional State, Ethiopia.

A total of blood samples were collected from. Session 6: Pathology T16 - Clinical and pathological aspects of dourine in Mongolia and therapeutic interventions Banzragch BATTUR - Mongolian University of Life Science, Mongolia T17 - Comparative clinico-pathological observations in young Zebu (Bos indicus) cattle experimentally infected with Trypanosoma vivax isolates from tsetse infested and non-tsetse areas of Northwest Ethiopia.

Biorepositories for biological samples have increasingly become very important in supporting biomedical research since the s. The Kenya Trypanosomiasis Research Institute Cryo‐bank for trypanosomes and their vectors was established in the s with the aim of providing research materials to scientists.

Over trypanosome isolates have been collected and stored in dewars under liquid. The most important trypanosome species affecting livestock in Ethiopia are Trypanosoma congolense, Trypanosoma vivax and Trypanosoma brucei, in cattle, sheep and goats, Trypanosoma evansi in camels and Trypanosoma equiperdiumin horses (Wondwesen et al., ).

The trypanosome caught the loss in. In Ethiopia, Trypanosomosis is widespread in domestic livestock in the Western, South and South-western lowland regions and the associated river systems i.e. Abay, Ghibe Omo and Baro/ Akobo Abebe and Jobre [8]. Currently aboutKm2 areas of the. Background. Trypanosoma (T.) evansi is a dyskinetoplastic variant of that has gained the ability to be transmitted by all sorts of biting can be divided into type A, which is the most abundant and found in Africa, Asia and Latin America and type B, which has so far been isolated only from Kenyan dromedary camels.

This study aimed at the isolation and the genetic and. However, cross-reactions between different trypanosome species do occur. Ashkar and Ochilo () found that more than 85 percent of cattle infected with T.

vivax or T. congolense reacted with T. brucei antigen in the IFAT (Table 3). When sera were tested against all three pathogenic trypanosome species, between 45 and 66 percent of sera. The three trypanocides used to control tsetse-transmitted trypanosomiasis in domestic animals in Africa have been in use for over 40 years and, not surprisingly, resistance of trypanosomes to these drugs has emerged.

Because of the relatively limited market in Africa and the high costs of developing and licensing new drugs, international pharmaceutical companies have shown little interest in. It Trypanosomosis is one of the major impediments to livestock development and agricultural production in Ethiopia.

It is cyclically transmitted by tsetse flies but also mechanically tsetse flies and by other biting flies. A cross sectional study was conducted in Botor tolay district of Oromia Regional State from November to may to determine the prevalence of trypanosomosis and.

African animal trypanosomosis, transmitted cyclically by tsetse flies or mechanically by other biting flies, causes serious inflictions to livestock health. This study investigates the extent of non-tsetse transmitted animal trypanosomosis (NTTAT) by Trypanosoma (T.) evansi and T.

vivax in domestic animals in the tsetse-free regions of Northern Ethiopia, Afar and Tigray. A cross sectional. Sixteen young (aged between 6 and 12 months) Zebu cattle (Bos indicus), purchased from a trypanosome-free area and confirmed to be trypanosome-negative, were randomly assigned into four groups of four animals.

Groups 1, 2 and 3 were infected with an isolate from a tsetse infested or one of two isolates from a non-tsetse infested area, and group. trypanosome species affecting livestock in Ethiopia are from the capital city, Addis Ababa. The area has an Trypanosoma congolense, Trypanosoma vivaxand altitude ranging from to m a.s.l.

with mean Trypanosoma brucei, in cattle, sheep and goat, annual rainfall of mm and mean annual. Herd A was a dairy herd of approximately Herd B was an isolated herd of 32 and contained both dairy and beef breeds.

Blood samples were collected from all animals in Herd A during July and August on two successive years. Samples were collected from Herd B at monthly intervals.

trypanosomes and trypanosomiasis Posted By Jin Yong Media TEXT ID adb6a Online PDF Ebook Epub Library cycles and some an area wide tsetse fly and cattle trypanosomosis survey was conducted in ethiopia groups of mice and pigs were experimentally exposed to g m morsitans. Methods.

Trypanosomes isolated from the urethral tract of a stallion with suspected dourine, were directly cultivated using soft agarose media at 37 °C in 5 % CO molecular characterization, 18S ribosomal RNA (rRNA) gene, the internal transcribed spacer (ITS) and 8 maxicircle DNA regions were amplified by a PCR and their sequences were determined.

~~ Book Trypanosomes And Trypanosomiasis ~~ Uploaded By Jir. Akagawa, this new volume written by experts in the field of trypanosome research covers every aspect of trypanosome vector host biology it is a must read for basic researchers working with trypanosomes and related organisms infection and drug development as well as.

Blood samples collected randomly from cattle were examined for presence of trypanosomes using the buffy coat technique and Giemsa thin blood smears.

Overall, 48 animals were found infected: 39 (81 percent) with Trypanosoma vivax, 7 (15 percent) with T. congolense and 2 (4 percent) with T.

brucei.Application of field methods to assess isometamidium resistance of trypanosomes in cattle in western Ethiopia. Acta Tropica, 90 (2): In this study, four stocks of Trypanosoma congolense, originally isolated from cattle in the southwest of Ethiopia (Ghibe, Isolation and characterization of gangliosides from Trypanosoma brucei.T1 - Molecular detection of trypanosomes in cattle in South America and genetic diversity of Trypanosoma evansi based on expression-site-associated gene 6.

AU - Mekata, Hirohisa. AU - Konnai, Satoru. AU - Witola, William H. AU - Inoue, Noboru. AU - Onuma, Misao. AU - Ohashi, Kazuhiko. PY - / Y1 - /

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